
READ ME File For 'MST data'

Dataset DOI: https://doi.org/10.5258/SOTON/D2547

ReadMe Author: Jacob Valentine, https://orcid.org/0009-0004-7956-733X

This dataset supports the thesis entitled "Using RTHS/SICLOPPS to identify inhibitors of the c-MYC/MAX interaction, and developing next generation sequencing approaches to SICLOPPS"
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2023

DESCRIPTION OF THE DATA
This data set consists of Microscale Thermophoresis data which was used to determine the affinity of peptides for the targets they were screened against. It consists of excel exports of data from the
proprietary NanoTemper software on the MST machine used to acquire it, and PRISM files (which require GraphPad PRISM to open) which used this data to determine the proper binding constants to assign to 
each peptide. Files with the same name but an addended FB are simply two separate representations of the same data (the raw FNorm data and it's fraction bound (FB) estimated respresentation). Data sets cover
HIF1a, HIF1b and HIF2a, and small titles in the "contains" and "date of collection" sections show which files correspond to which protein. Original data files which require the NanoTemper Software suite to 
open and read can be found on the associated laptop, Floor 4 of Building 30, University of Southampton Highfield Campus Chemistry.

This dataset contains:
Title: HIF1a MST
CLLFVY test.xlsx
SICLOPPS singlicates 1 to 12.xlsx
SICLOPPS singlicates 13 to 18.xlsx
SICLOPPS singlicates 19 to 23.xlsx
HIF CX5 singlicates.pzfx
Top 5 peptide 12pt triplicates.xlsx
Top 5 peptide 12pt triplicates FB.xlsx
Top 5 peptide 12pt triplicates.pzfx
Top 5 peptide 12pt triplicates FB.pzfx
Final 16pt triplicates.xlsx
Final 16pt triplicates FB.xlsx
CLLFCL 1a 2a 1b.pzfx (contains final triplicates for each protein)
CRTYIL 1a 2a 1b.pzfx (contains final triplicates for each protein)
CRYLIL 1a 2a 1b.pzfx (contains final triplicates for each protein)

Title: HIF1b MST
HIF1b 8pt sinligcate tests A.xlsx
HIF1b 8pt sinligcate tests B.xlsx
HIF1b 8pt sinligcate tests C.xlsx
HIF1b 8pt sinligcate tests D.xlsx
HIF1b 8pt singlicate tests.pzfx
HIF1b 1a lead compound tests FB.xlsx
HIF1b dominant tests FB.xlsx
HIF1b triplicate tests FB.pzfx

Title: HIF2a MST
HIF2a triplicate tests FB.xlsx
HIF2a triplicate tests FB.pzfx

Date of data collection: 
Title: HIF1a MST
2021-08-12
2021-08-12 to 2021-08-15
2021-08-21
2021-08-21
N/A Data analysis not acquisition
2021-08-22
2021-08-22
N/A Data analysis not acquisition
N/A Data analysis not acquisition
2021-11-12 to 2021-11-28
2021-11-12 to 2021-11-28
N/A Data analysis not acquisition
N/A Data analysis not acquisition
N/A Data analysis not acquisition

Title: HIF1b MST
2021-09-13
2021-10-01
2021-10-01
2021-10-02
N/A Data analysis not acquisition
2021-11-06 and 2021-11-08
2021-11-22
N/A Data analysis not acquisition

Title: HIF2a MST
2021-11-06
N/A Data analysis not acquisition

Information about geographic location of data collection: Collected in Building 30, Highfield Campus, University of Southampton

